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pca t int g  (Addgene inc)


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    Structured Review

    Addgene inc pca t int g
    Pca T Int G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pca+t+int+g/pCA-T-intron-G+(Plasmid+%2336887)/pmc04587569-25-11-12
    Average 85 stars, based on 1 article reviews
    pca t int g - by Bioz Stars, 2026-09
    85/100 stars

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    Modification:

    Article Title: Viral-genetic tracing of the input–output organization of a central norepinephrine circuit
    Article Snippet: We serially inserted into this pBluescript the following DNA fragments by using the unique restriction sites. (1) HindIII-SpeI fragment containing the WPRE and human growth hormone polyA signal obtained from pAAV-TRE-HTG (addgene#27437) . (2) MluI/KpnI fragment containing the CAG promoter. .. To make this fragment, we sub-cloned PstI-XmaI flanked CAG promoter from pCA-T-int-G (addgene# 36887) into a modified pBluescript II SK vector that only contains MluI-PstI-XmaI-KpnI recognition sequence in the cloning site. (3) AscI/SalI fragment containing coding sequence of G or TC cassette, obtained from pAAV CAG-FLEx loxP -G (addgene#48333) or pAAV CAG-FLEx loxP -TC (addgene# 48332) . .. The assembled cassettes were sub-cloned into pAAV-MCS (AAV helper free system, Stratagene, Cat# 240071-12) using the NotI sites.

    Plasmid Preparation:

    Article Title: Viral-genetic tracing of the input–output organization of a central norepinephrine circuit
    Article Snippet: We serially inserted into this pBluescript the following DNA fragments by using the unique restriction sites. (1) HindIII-SpeI fragment containing the WPRE and human growth hormone polyA signal obtained from pAAV-TRE-HTG (addgene#27437) . (2) MluI/KpnI fragment containing the CAG promoter. .. To make this fragment, we sub-cloned PstI-XmaI flanked CAG promoter from pCA-T-int-G (addgene# 36887) into a modified pBluescript II SK vector that only contains MluI-PstI-XmaI-KpnI recognition sequence in the cloning site. (3) AscI/SalI fragment containing coding sequence of G or TC cassette, obtained from pAAV CAG-FLEx loxP -G (addgene#48333) or pAAV CAG-FLEx loxP -TC (addgene# 48332) . .. The assembled cassettes were sub-cloned into pAAV-MCS (AAV helper free system, Stratagene, Cat# 240071-12) using the NotI sites.

    Sequencing:

    Article Title: Viral-genetic tracing of the input–output organization of a central norepinephrine circuit
    Article Snippet: We serially inserted into this pBluescript the following DNA fragments by using the unique restriction sites. (1) HindIII-SpeI fragment containing the WPRE and human growth hormone polyA signal obtained from pAAV-TRE-HTG (addgene#27437) . (2) MluI/KpnI fragment containing the CAG promoter. .. To make this fragment, we sub-cloned PstI-XmaI flanked CAG promoter from pCA-T-int-G (addgene# 36887) into a modified pBluescript II SK vector that only contains MluI-PstI-XmaI-KpnI recognition sequence in the cloning site. (3) AscI/SalI fragment containing coding sequence of G or TC cassette, obtained from pAAV CAG-FLEx loxP -G (addgene#48333) or pAAV CAG-FLEx loxP -TC (addgene# 48332) . .. The assembled cassettes were sub-cloned into pAAV-MCS (AAV helper free system, Stratagene, Cat# 240071-12) using the NotI sites.

    Cloning:

    Article Title: Viral-genetic tracing of the input–output organization of a central norepinephrine circuit
    Article Snippet: We serially inserted into this pBluescript the following DNA fragments by using the unique restriction sites. (1) HindIII-SpeI fragment containing the WPRE and human growth hormone polyA signal obtained from pAAV-TRE-HTG (addgene#27437) . (2) MluI/KpnI fragment containing the CAG promoter. .. To make this fragment, we sub-cloned PstI-XmaI flanked CAG promoter from pCA-T-int-G (addgene# 36887) into a modified pBluescript II SK vector that only contains MluI-PstI-XmaI-KpnI recognition sequence in the cloning site. (3) AscI/SalI fragment containing coding sequence of G or TC cassette, obtained from pAAV CAG-FLEx loxP -G (addgene#48333) or pAAV CAG-FLEx loxP -TC (addgene# 48332) . .. The assembled cassettes were sub-cloned into pAAV-MCS (AAV helper free system, Stratagene, Cat# 240071-12) using the NotI sites.



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    85
    Addgene inc pca t int g
    Pca T Int G, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pca+t+int+g/pCA-T-intron-G+(Plasmid+%2336887)/pmc04587569-25-11-12
    Average 85 stars, based on 1 article reviews
    pca t int g - by Bioz Stars, 2026-09
    85/100 stars
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